General Biology 2: Recombinant DNA

Subject: Biology 2

Topic: Recombinant DNA

Difficulty: Hard

Questions: 5

Passing Score: 70%

Question #1 1 point

A scientist wants to create a bacterium that can produce human growth hormone. She uses
"molecular scissors" to open a plasmid. What is the technical name for these scissors and their
specific action?

A: Ligase; they join DNA segments together.

B: Vectors; they transport the DNA

C: Restriction enzymes; they cut DNA at specific target sequences.

D: Ribosomes; they decode the RNA.

Question #2 1 point

During the process of creating recombinant DNA, why are bacterial plasmids often selected as
vectors?

A: They are large and can hold an entire organism’s genome.

B: They are small, circular, and can easily carry and propagate extra spliced DNA.

C: They are only found in human cells.

D: They prevent the host cell from developing drug resistance.

Question #3 1 point

In the "Ligation" step of genetic engineering, what is the final outcome of joining a gene of
interest with a bacterial plasmid?

A: The creation of a genomic library.

B: The immediate extinction of the host cell.

C: The formation of recombinant DNA from two different sources.

D: The determination of the exact order of nucleotides.

Question #4 1 point

How does "Classical Breeding" differ from "Recombinant DNA Technology"?

A: Classical breeding uses laboratory enzymes to splice genes.

B: Classical breeding involves mating members of a species, while Recombinant DNA allows for splicing DNA from different species.

C: Recombinant DNA technology is an ancient practice used for centuries.

D: There is no difference; both terms refer to cloning.

Question #5 1 point

When using genetic engineering in industry, why are transformed microorganisms grown in
large bioreactors?

A: To observe the evolutionary history of the bacteria.

B: To mass-produce and then purify specific proteins coded by the inserted genes.

C: To prevent the bacteria from developing new traits

D: To sequence the entire genome of the human family tree.

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